Digestive System (including glands, oral cavity & alimentary tract)

What are the best of preparing the human tongue for staining? I am studying the nerves in the tongue and i was hoping if you can help me by telling me what are the best staining to use? And how thick should the cross-section (slice) of the cadaver tongue?

Thu, 2011-06-23 12:48 — Anonymous

Your question is a very good one, and it took a lot of collaboration to give you some help!

To stain nerves, use Golgi or Weigert’s stains; alternatives may be Mallory-Azan, Carmine, Cajal.

Another colleague (Dr. Rick Puzdrowski) suggested clearing the tongue with trypsin and use Sudan Black to stain the nerves as a whole mount. He also suggested that you “process the tissue and embed it in paraplast, section at 10 microns, and stain the sections with Sudan Black.”

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Medical Student

Digestive System (including glands, oral cavity & alimentary tract)

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Digestive System (including glands, oral cavity & alimentary tract)

What are the best of preparing the human tongue for staining? I am studying the nerves in the tongue and i was hoping if you can help me by telling me what are the best staining to use? And how thick should the cross-section (slice) of the cadaver tongue?